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Published on: November 21, 2023
On-capillary chemiluminescence detection for capillary electrophoresis with a single capillary
Cheng-Jie Zhang1, Xing-Zheng Wu
1Department of Materials Science and Engineering, School of Engineering, University of Fukui, Japan.
Abstract:
On-capillary chemiluminescence detection for capillary electrophoresis with a single capillary was reported. A hole (about 30 microm diameter) was made on the capillary wall at about 50.5 cm from the inlet end. Hydrogen peroxide solution could enter the capillary from the hole, and mixed with luminol and copper(II) to produce chemiluminescence. The chemiluminescence was detected by a PMT under the hole. Several factors that influenced chemiluminescence intensity were investigated. The detection limits for luminol and N-(4-aminolbutyl)-N-ethylisoluminol (ABEI) were 1 x 10(-11) and 2 x 10(-10) mol L(-1), respectively. The method features simple construction and no dead volume.
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