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Evaluation of the Roche Septi-Chek AFB system for recovery of mycobacteria
R F D'Amato1, H D Isenberg, L Hochstein
1Department of Pathology, Catholic Medical Center of Brooklyn & Queens, Inc., Jamaica, New York 11432-3723.
Abstract:
The Septi-Chek AFB system for the recovery of mycobacteria from clinical specimens was compared with a conventional approach using Lowenstein-Jensen and Middlebrook 7H11 agars. A total of 1,532 clinical specimens were analyzed; 132 yielded mycobacteria. Mycobacterium tuberculosis and Mycobacterium avium complexes were the predominant isolates. With the conventional combination of Lowenstein-Jensen and 7H11 agars, 75.8% of the isolates were recovered; the Septi-Chek AFB allowed recovery of 100% of the isolates. Septi-Chek AFB required less time for the detection of mycobacteria than did the conventional media.