Projections from the posteroventral cochlear nucleus to the superior olivary complex in guinea pig: light and EM...

The efferent neural projections from posteroventral cochlear nucleus to the superior olivary complex in guinea pig were examined with the Phaseolus vulgaris-leucoagglutinin anterograde tract-tracing method. Light microscopic analysis demonstrated that the posteroventral cochlear nucleus of guinea pig bilaterally projects to the superior para-olivary nucleus and the rostral, medioventral, and lateroventral peri-olivary regions. Ipsilaterally, the posteroventral cochlear nucleus projects to the lateral superior olive, the caudal peri-olivary region, and areas immediately surrounding the capsule of the lateral superior olive. Contralaterally, the posteroventral cochlear nucleus projects to the medial nucleus of the trapezoid body. All of these projection axons travel in the trapezoid body and their terminals make, primarily, en passant endings upon their targets. Exclusively within the contralateral medial nucleus of the trapezoid body, some neurons terminate also in calyceal endings. The assumption that immunolabeled structures observed with light microscopy made actual synaptic contact in their respective target areas was confirmed with electron microscopy. With postembedding immunocytochemical procedures applied to thin sections, the electron microscope revealed labeled synaptic vesicles and pre- and postsynaptic membrane specializations. The projection pattern from posteroventral cochlear nucleus was found to be topographically organized in three distinct regions. The more dorsally located neurons of the posteroventral cochlear nucleus terminated dorsomedially in the ipsilateral lateral superior olive, ventromedially in the contralateral superior para-olivary nucleus, and medially in the contralateral medioventral peri-olivary region. The more ventrally located neurons of the posteroventral cochlear nucleus terminated dorsolaterally in the ipsilateral lateral superior olive, dorsolaterally in the contralateral superior para-olivary nucleus, and laterally in the contralateral medioventral peri-olivary region.