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Related Experiment Video

Updated: Jul 14, 2026

Isolating Lymphocytes from the Mouse Small Intestinal Immune System

Isolating Lymphocytes from the Mouse Small Intestinal Immune System

Published on: February 28, 2018

Lymphatic development in mouse small intestine.

Kyung Eun Kim¹, Hoon-Ki Sung, Gou Young Koh

¹National Research Laboratory of Vascular Biology and Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Korea.

Developmental Dynamics : an Official Publication of the American Association of Anatomists

|June 20, 2007

Summary

Embryonic development of intestinal lymphatic vessels was studied in mice. Lymphatic vessels form through branching from the mesentery, with mature vasculature present before birth.

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Last Updated: Jul 14, 2026

Isolating Lymphocytes from the Mouse Small Intestinal Immune System

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Published on: February 28, 2018

Long-Term Catheterization of the Intestinal Lymph Trunk and Collection of Lymph in Neonatal Pigs

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Isolation and Flow Cytometric Characterization of Murine Small Intestinal Lymphocytes

Isolation and Flow Cytometric Characterization of Murine Small Intestinal Lymphocytes

Published on: May 8, 2016

Area of Science:

Developmental Biology
Vascular Biology
Gastroenterology

Background:

Intestinal lymphatic vessels are crucial for lipid absorption and transport.
The embryonic development of intestinal lymphatic vasculature remains largely uncharacterized.

Purpose of the Study:

To investigate the embryonic development of lymphatic and blood vasculature in the mouse small intestine.
To identify the origin and formation process of intestinal lymphatic vessels during embryonic development.

Main Methods:

Immunostaining of mouse embryonic intestinal tissues using molecular markers for lymphatic endothelial cells (LYVE-1, VEGFR3, Prox-1, podoplanin).
Analysis of vascular development at different embryonic stages (E13.5-E17.5).

Main Results:

Immature lymphatics appeared in the mesentery around E13.5-E14.5, with organized plexuses and capillaries forming in the intestinal tube and villi by E17.5.
Intestinal lymphatic vessels develop via active branching from mesenteric lymphatics, involving VEGFR3 activation and LYVE-1+ macrophages.
Unlike blood vessels, intestinal lymphatics do not originate from the intestinal mesoderm but extend from the mesentery.

Conclusions:

Intestinal lymphatic vasculature forms after blood vessel development but matures before birth.
This study elucidates the temporal and spatial dynamics of intestinal lymphatic development in mouse embryos.