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Updated: Jul 14, 2026

In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA
Published on: May 21, 2015
Molecular detection and identification of Synergistes phylotypes in primary endodontic infections
1Department of Endodontics, Estácio de Sá University, Rio de Janeiro, Brazil. siqueira@estacio.br
Aim:
Uncultivated phylotypes from the Synergistes group have recently emerged as suspected endodontic pathogens. This study aimed to investigate the presence and identity of these bacteria in primary endodontic infections using a 16S rRNA gene-based group-specific heminested polymerase chain reaction (PCR) protocol.
Materials And Methods:
Genomic DNA was isolated directly from clinical samples and used as templates for PCR. Amplicons from positive specimens were sequenced and phylogenetically analyzed to determine species identity.
Results:
Overall, about one-third of the samples harbored Synergistes bacteria. The following phylotypes were disclosed: oral clones W028, BA121/P4G_18 P1, W090, BH017 and E3_33.
Conclusions:
These results demonstrated that as-yet uncultivated Synergistes phylotypes are present in the endodontic microbiota and a role in causation of apical periodontitis is suggested though remains unproven.
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