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Related Experiment Videos

Microbial flora in semen during in vitro fertilization.

C Huyser1, F L Fourie, M Oosthuizen

  • 1Department of Obstetrics and Gynaecology, University of Pretoria, Pretoria, RSA.

Journal of in Vitro Fertilization and Embryo Transfer : IVF
|October 1, 1991
PubMed
Summary

Antimicrobial treatment and semen processing significantly reduced microbial contamination in semen samples. Semen preparation was more effective, highlighting its importance for in vitro fertilization success.

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Area of Science:

  • Reproductive Medicine
  • Microbiology
  • In Vitro Fertilization

Background:

  • Microbial contamination in semen can negatively impact in vitro fertilization (IVF) outcomes.
  • Understanding contamination rates and effective removal methods is crucial for improving IVF success.

Purpose of the Study:

  • To determine the bacterial and fungal contamination rates in semen before and after antibiotic treatment.
  • To assess the efficacy of semen preparation techniques (wash and swim-up) in reducing microbial load.
  • To evaluate the impact of microbial contamination on oocyte fertilization and development.

Main Methods:

  • Retrospective analysis of 183 semen samples from men in an IVF program.
  • Evaluation of microbial contamination before and after antibiotic treatment.

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  • Assessment of semen processing (wash and swim-up) in 102 samples.
  • Microscopic examination of oocytes for fertilization and development.

    • Main Results:

    • Antimicrobial treatment decreased pathogen incidence by 16.3% (P < 0.0001).
    • Semen processing effectively removed microbial contaminants from 57.4% of samples (P < 0.0001).
    • Infected culture media led to oocyte degeneration, with no fertilization or pronuclei observed.

    Conclusions:

    • Both antibiotic treatment and semen processing are effective in reducing microbial contamination.
    • Semen preparation methods are highly effective in eliminating microorganisms from semen samples.
    • Microbial contamination poses a significant threat to oocyte fertilization and embryonic development in IVF.