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Preparation of Intact Bovine Tail Intervertebral Discs for Organ Culture

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[In vitro studies on tissue engineered intervertebral disc].

Chao Zhang¹, Dike Ruan, Rongfeng Zhang

¹Departmant of Orthopaedics and Traumatology, Navy General Hospital, Beijing, 100037, P. R China. zcmail2002@yahoo.com.cn

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi = Zhongguo Xiufu Chongjian Waike Zazhi = Chinese Journal of Reparative and Reconstructive Surgery

|June 21, 2007

Summary

Tissue engineered intervertebral discs show promise for regeneration. Human fetal disc cells proliferated within a porous scaffold, supporting potential applications in treating disc degenerative diseases.

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Published on: February 14, 2021

Area of Science:

Biomaterials Science
Regenerative Medicine
Cell Biology

Background:

Intervertebral disc degeneration is a significant cause of back pain.
Current treatments for disc degeneration have limitations.
Tissue engineering offers a potential solution for disc regeneration.

Purpose of the Study:

To assess the feasibility of using tissue-engineered intervertebral discs for disc regeneration.
To evaluate the viability and proliferation of human fetal disc cells within a novel scaffold.

Main Methods:

Fabrication of a 3D porous poly(lactic-co-glycolic acid) (PLGA) scaffold using temperature-induced phase separation.
Isolation and in vitro culture of human fetal disc cells.
Seeding of PKH-26 labeled disc cells into the scaffold and assessment of proliferation via MTT assay, fluorescence microscopy, and SEM.

Main Results:

A porous scaffold with a regular arrangement and microtubule orientation (50-300 micrometers) was successfully fabricated.
Human fetal disc cells exhibited polygonal shapes in culture and demonstrated viability and proliferation within the porous scaffold.
MTT uptake and fluorescent microscopy confirmed cell survival and proliferation within the engineered construct.

Conclusions:

The study demonstrates the potential of tissue-engineered intervertebral discs for regenerative applications.
The findings support the use of PLGA scaffolds seeded with human fetal disc cells for treating disc degenerative diseases.