Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Gene Therapy00:59

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be inserted. The...
Gene Therapy00:59

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be inserted. The...
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Task-Evoked Functional Activation and Coupling With CSF Flow Detected in the Human Brain With Ultrashort Echo Time fMRI at 7 T.

NMR in biomedicine·2026
Same author

Mapping cardiac and respiratory pulsations simultaneously with functional connectivity in the rat brain using zero echo time fMRI.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism·2026
Same author

Secondary thalamic neuroinflammation associates with disturbed corticothalamic connectivity in a model of severe traumatic brain injury in male rats-a longitudinal study.

Cerebral cortex (New York, N.Y. : 1991)·2026
Same author

Functional MRI following sensory stimulation in rat monosodium iodoacetate model of osteoarthritis pain as a tool for drug therapy discovery.

NeuroImage·2025
Same author

Multimodal approach to characterize surgically removed epileptogenic zone from patients with focal drug-resistant epilepsy: From operating room to wet lab.

Epilepsia open·2025
Same author

Standardization of preclinical methodologies for discovery and validation of circulating microRNA biomarkers for post-traumatic epileptogenesis - Lessons learned from the EpiBioS4Rx Project 1.

Epilepsy research·2025

Related Experiment Video

Updated: Jul 14, 2026

Ultrahigh Resolution Mouse Optical Coherence Tomography to Aid Intraocular Injection in Retinal Gene Therapy Research
10:10

Ultrahigh Resolution Mouse Optical Coherence Tomography to Aid Intraocular Injection in Retinal Gene Therapy Research

Published on: November 2, 2018

Non-invasive Imaging in Gene Therapy.

Jani Kristian Räty1, Timo Liimatainen, Minna Unelma Kaikkonen

  • 1Department of Biotechnology and Molecular Medicine, A.I. Virtanen Institute for Molecular Sciences, University of Kuopio, Kuopio, Finland.

Molecular Therapy : the Journal of the American Society of Gene Therapy
|June 21, 2007
PubMed
Summary

Non-invasive imaging techniques like MRI, SPECT, PET, and optical imaging are crucial for evaluating gene therapy vectors. Each method offers unique trade-offs in resolution, cost, and sensitivity for tracking gene delivery and expression in vivo.

More Related Videos

Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models
10:04

Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models

Published on: March 13, 2018

Molecular Imaging to Target Transplanted Muscle Progenitor Cells
09:24

Molecular Imaging to Target Transplanted Muscle Progenitor Cells

Published on: March 27, 2013

Related Experiment Videos

Last Updated: Jul 14, 2026

Ultrahigh Resolution Mouse Optical Coherence Tomography to Aid Intraocular Injection in Retinal Gene Therapy Research
10:10

Ultrahigh Resolution Mouse Optical Coherence Tomography to Aid Intraocular Injection in Retinal Gene Therapy Research

Published on: November 2, 2018

Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models
10:04

Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models

Published on: March 13, 2018

Molecular Imaging to Target Transplanted Muscle Progenitor Cells
09:24

Molecular Imaging to Target Transplanted Muscle Progenitor Cells

Published on: March 27, 2013

Area of Science:

  • Biomedical imaging
  • Gene therapy research
  • Molecular imaging techniques

Background:

  • Non-invasive imaging is essential for assessing gene delivery and transgene expression in vivo.
  • Current modalities like MRI, SPECT, PET, and optical imaging vary significantly in sensitivity, cost, and resolution.
  • Understanding these differences is key to selecting appropriate imaging methods for gene therapy development.

Purpose of the Study:

  • To review current non-invasive imaging methodologies for gene therapy.
  • To highlight recent advancements in gene therapy imaging.
  • To discuss future trends in the field of gene therapy imaging.

Main Methods:

  • Comparison of magnetic resonance imaging (MRI), single photon emission tomography (SPECT)/positron emission tomography (PET), and fluorescence/bioluminescence imaging.
  • Analysis of modality-specific characteristics: sensitivity, cost, resolution, and probe requirements.
  • Review of recent literature on imaging approaches in gene therapy.

Main Results:

  • MRI offers high resolution (~50 µm) but requires higher probe amounts.
  • Optical imaging is cost-effective but has lower resolution (3-5 mm).
  • SPECT/PET provide good resolution (1-2 mm) with high sensitivity and lower probe requirements.

Conclusions:

  • The choice of imaging modality significantly impacts the resolution and quantitation of gene therapy outcomes.
  • Rigorous in vivo experiments using appropriate imaging are vital for developing safer and more effective gene delivery vectors.
  • Continued advancements in imaging technologies will further enhance the evaluation and application of gene therapy.