Embryonic factors affecting outcome from single cryopreserved embryo transfer

Multiple pregnancy minimization by single embryo transfer is becoming more prevalent, but is less common in the case of cryopreserved embryos. This study defines embryonic characteristics in single cryopreserved embryo transfers associated with success rates equivalent to those achieved when transferring two cryopreserved embryos. In a retrospective analysis of 6916 cryopreserved day-2 embryo transfer procedures, transfer of two cryopreserved embryos resulted in higher clinical pregnancy rates when compared with transfer of a single thawed embryo but was also associated with elevated multiple pregnancy rates (26.7% in women under 36). Optimal outcome (implantation rate of 30.9%) from single cryopreserved embryo transfer (SCET) in women under 36 was associated with cryopreservation at the 4-cell stage, loss of fewer than two blastomeres and subsequent cleavage of at least two surviving blastomeres. In comparison, transfer of two cryopreserved embryos in women under 36 resulted in pregnancy and implantation rates of 25.5 and 16.1% respectively. Interestingly, in cryopreserved 4-cell stage embryos, loss of a single blastomere did not reduce implantation potential and cleavage of only a single post-thaw blastomere was not indicative of increased implantation potential. Establishment of these critical thresholds provides a rational basis for SCET.