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Related Concept Videos

Centrosome Duplication02:25

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
Centrosome Duplication02:25

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
Centrioles and Centrosomes01:13

Centrioles and Centrosomes

Most animal cells comprise a pair of centrioles together called a centrosome. The cell duplicates its centrosome and contains two centrosomes side-by-side, which begin to move apart during the prophase. As the centrosomes migrate to two different sides of the cell, microtubules start extending from each centrosome toward the other end. The mitotic spindle is composed of the centrosomes and their emerging microtubules.
Near the end of the prophase, also called late prophase or "prometaphase,"...
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
Renewal of Skin Epidermal Stem Cells01:12

Renewal of Skin Epidermal Stem Cells

The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular cells,...

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Related Experiment Video

Updated: Jul 14, 2026

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations
07:14

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Published on: September 20, 2019

Stem cell self-renewal: centrosomes on the move.

Clemens Cabernard1, Chris Q Doe

  • 1Institute of Neuroscience, HHMI, University of Oregon, Eugene, Oregon 97403, USA.

Current Biology : CB
|June 21, 2007
PubMed
Summary

Centrosome asymmetry is linked to stem cell self-renewal in Drosophila. Equalizing centrosomes disrupts the asymmetric cell division crucial for stem cell function.

Area of Science:

  • Cell Biology
  • Developmental Biology
  • Stem Cell Biology

Background:

  • Stem cell self-renewal is vital for tissue homeostasis and development.
  • Asymmetric cell division is a key mechanism for maintaining stem cell populations.
  • The role of centrosomes in regulating stem cell division and self-renewal requires further elucidation.

Purpose of the Study:

  • To investigate the correlation between centrosome asymmetry and stem cell self-renewal in Drosophila.
  • To determine the impact of equalizing centrosomes on asymmetric cell division in stem cells.

Main Methods:

  • Analysis of centrosome structure and distribution in Drosophila neural and germline stem cells.
  • Experimental manipulation to equalize centrosome size and number.

More Related Videos

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
09:39

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Published on: December 20, 2014

Imaging Centrosomes in Fly Testes
09:41

Imaging Centrosomes in Fly Testes

Published on: September 20, 2013

Related Experiment Videos

Last Updated: Jul 14, 2026

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations
07:14

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Published on: September 20, 2019

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
09:39

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Published on: December 20, 2014

Imaging Centrosomes in Fly Testes
09:41

Imaging Centrosomes in Fly Testes

Published on: September 20, 2013

  • Observation and quantification of cell division patterns and stem cell behavior.

    • Main Results:

    • A significant correlation was observed between centrosome asymmetry and the self-renewal capacity of Drosophila neural and germline stem cells.
    • Experimental equalization of centrosomes led to the disruption of asymmetric cell division.
    • Disruption of asymmetric division resulted in altered stem cell behavior and potential loss of self-renewal.

    Conclusions:

    • Centrosome asymmetry is a critical regulator of self-renewal in Drosophila stem cells.
    • Maintaining centrosome asymmetry is essential for proper asymmetric cell division.
    • These findings highlight the centrosome as a key player in stem cell biology and asymmetric cell division.