Related Experiment Video
Updated: Jul 14, 2026
12:42
Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo
Published on: January 7, 2019
Engineering functional protein interfaces for immunologically modified field effect transistor (ImmunoFET) by
Edward Eteshola1, Matthew T Keener, Mark Elias
1Department of Biomedical Engineering, The Ohio State University, Columbus, OH 43210, USA.
Journal of the Royal Society, Interface
|June 21, 2007
Summary
Researchers engineered biomolecule interactions on field-effect transistor (FET) sensors for improved biosensing. They developed methods to orient receptor proteins on FET surfaces, enhancing the performance of these hybrid biological-synthetic devices.
Area of Science:
- Biomolecular Engineering
- Nanotechnology
- Biosensor Development
Background:
- Analyte-receptor interactions at solid-liquid interfaces are crucial for hybrid biological-synthetic sensor devices.
- Field-effect transistor (FET) sensors offer a platform for detecting biomolecular interactions.
Purpose of the Study:
- To engineer the sensing interface of biochemically modified FET sensors (BioFETs) using protein engineering.
- To achieve oriented attachment and controlled interactions of receptor proteins on FET surfaces.
Main Methods:
- Direct adsorption and self-assembled monolayers for tethering receptor proteins to FET gates.
- Phage display to identify peptides recognizing SiO2 surfaces for oriented protein attachment.
- Protein engineering via scanning circular permutagenesis to control protein topography and functional domain positioning.
- Identification of single-chain fragment variables (scFvs) as potential sensor receptors.
Main Results:
- Demonstrated biochemical and electrical functionality of modified BioFET sensors.
- Developed methods for oriented immobilization of receptor proteins on FET sensing surfaces.
- Engineered protein variants with altered topography for controlled surface interactions.
Conclusions:
- Protein engineering strategies can effectively control biomolecule-surface interactions in BioFETs.
- Oriented immobilization of receptors enhances the performance of biosensor devices.
- These advancements are key to developing next-generation hybrid biological-synthetic sensors.
