[Damage effects of shock lymph on the pulmonary micro-vascular endothelial cells of rats]

The model of serious hemorrhagic shock was established under the condition of asepsis and mesentery lymph was taken out. As control, normal mesentery lymph fluid, normal portal vein blood, and shock portal vein blood of rats were taken out. The primary pulmonary microvascular endothelial cells (PMVECs) of passages 3 were treated by different treatment factors, respectively. The morphology and ultrastructure changes of PMVECs were observed under optics microscope, transmit electronic microscope and scan electronic microscope. The cells proliferation under different final concentrations of shock lymph and normal lymph were measured with MTT method. The cell cycle arrest was analyzed by flow cytometry and the DNA of cell nucleus was analyzed by electrophoresis. The results showed that shock lymph played a role in damaging PMVECs. The contracted cells and condensed nucleus were found, and apoptosic bodies were observed under a transmission electron microscope. The proliferation of PMVECs was decreased when the concentration of shock lymph increased to some extent and showed statistic significance compared with normal lymph group. The cells cocultured with shocking lymph fluid at 4% final concentration showed that G0-G1 cell population was higher and the proportion of S+G2-M cell population was lower than that of other groups, and the DNA ladder was observed in electrophoresis of cell nucleus DNA at same time. The results demonstrated that shock lymph could damage the morphology and ultra-structure of PMVECs, reduce the cells proliferation, interfere with cell cycle, and induce the apoptosis.