[Applications of molecularly imprinted column for chiral separation by high performance liquid chromatography and...

The molecularly imprinted polymer (MIP) monolithic column was firstly in situ polymerized in a traditional stainless-steel chromatographic column by using methylacrylic acid and S-Tröger's base as the functional monomer and imprinted template molecules, respectively. The effects of the content of acetic acid and water in mobile phase on the separation were systematically investigated. The fast enantioseparation of the racemate on monolithic column was successfully realized by stepwise gradient elution. The MIP monolithic capillary column was also synthesized in a fused-silica capillary by in situ polymerization with 2-( dimethylamino) ethyl methacrylate (DAMA) as functional monomer. The enantioseparation of 1,1'-bi-2-naphthol (BNL) on the obtained MIP capillary column was achieved in capillary electrochromatography (CEC). The results indicated that other acidic template can be imprinted by using basic DAMA as functional monomer, which enlarge the application fields of imprinting molecules.