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Related Concept Videos

High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

3.1K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
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Gas Chromatography: Types of Columns and Stationary Phases01:17

Gas Chromatography: Types of Columns and Stationary Phases

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Gas chromatography (GC) relies on stationary phases to separate and analyze components in a sample. There are two main types of stationary phases: liquid and solid. Liquid stationary phases are non-volatile, thermally stable, and chemically inert liquids coated onto the column. Solid stationary phases are particles of adsorbent material, such as silica gel or molecular sieves.
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
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Silica Gel Column Chromatography: Overview01:10

Silica Gel Column Chromatography: Overview

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Silica gel column chromatography is a technique for separating compounds using a column packed with silica gel as the stationary phase. This method relies on differences in the polarity of compounds. Based on their polarities, compounds move between the stationary phase (silica gel) and the mobile phase (the solvent), forming discrete bands in the column.
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
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High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

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In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
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Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

1.6K
In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
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High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

2.8K
High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
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Related Experiment Video

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Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach
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[Silica based stationary phases for high performance liquid chromatography].

Shengxiang Jiang1, Xia Liu

  • 1Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China. sxjiang@lzb.ac.cn

Se Pu = Chinese Journal of Chromatography
|June 22, 2007
PubMed
Summary

This review covers silica-based packing materials for high-performance liquid chromatography (HPLC). It synthesizes laboratory findings and existing literature for advancements in HPLC separation technologies.

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Area of Science:

  • Analytical Chemistry
  • Materials Science

Context:

  • High-performance liquid chromatography (HPLC) is a critical analytical technique.
  • Silica-based materials are widely used as stationary phases in HPLC.

Purpose:

  • To review silica-based packing materials for HPLC.
  • To consolidate laboratory research with existing literature.
  • To provide a comprehensive overview of advancements in the field.

Summary:

  • The review synthesizes 157 references on silica-based packing materials for HPLC.
  • It integrates findings from the authors' laboratory work.
  • Key developments and applications of these materials are discussed.

Impact:

  • Provides a valuable resource for researchers in chromatography.
  • Highlights current trends and future directions in HPLC material development.
  • Aids in the selection and design of optimal stationary phases for specific separations.