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A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
Purification of dibenzothiophene monooxygenase from a recombinant Escherichia coli
Nidhi Gupta1, D K Adhikari, T Stobdan
1Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi, 110016, India.
Biotechnology Letters
|June 22, 2007
Abstract:
Dibenzothiophene monooxygenase is the first enzyme involved in the degradation of dibenzothiophene. This gene was expressed via the pET28a vector in E. coli and was purified in a single step using affinity chromatography. The protein was purified 39-fold with a specific activity of 38 U/mg.

