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[Technique of macroporous resin for tartary buckwheat total flavonoids]
1College of Food Science and Engineering, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100, China. hheather@etang.com
Objective:
To investigatre appropriate macroporous resins and the optimal technological parameters of the purification process of tartary buckwheat total flavonoids.
Method:
Static and dynamic adsorption-desorption methods were adopted, and evaluated for separating efficiency by macroporous resins absorption rates, desorption rates.
Result:
The DM -2 macroporous resin had the best separating efficiency. The best absorbed condition is H2O as solvent of extracts, diameter vs height: 1: 10, pH 3 - 4, absorption power: 3.0 mL x min(-1). The optimum adsorption condition is the volume of 50% ethanol 80 mL (approximately 5 BV (resin bed volume) as eluting solvent, pH 8, the desorption power: 3.0 mL x min(-1).
Conclusion:
This technology is simple, quick and cost-effective, which is suitable for industrialization.