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Related Experiment Videos

Failed fertilization after ICSI and spermiogenic defects.

Mohammad H Nasr-Esfahani1, Shahnaz Razavi, Marzeyeh Tavalaee

  • 1Department of Embryology and Andrology, Royan Institute, Tehran, Iran. mh_nasr@med.mui.ac.ir

Fertility and Sterility
|June 23, 2007
PubMed
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Protamine deficiency significantly impacts fertilization rates in intracytoplasmic sperm injection (ICSI) cycles. This sperm DNA issue is more critical than sperm morphology or acrosin activity for successful fertilization.

Area of Science:

  • Reproductive Biology
  • Andrology
  • Infertility Research

Background:

  • Late spermiogenic events are crucial for male fertility.
  • Sperm DNA integrity, including histone-protamine replacement, is vital for successful fertilization.
  • Intracytoplasmic sperm injection (ICSI) bypasses natural fertilization barriers, but sperm quality remains critical.

Purpose of the Study:

  • To investigate the association between late spermiogenic events and fertilization rates in ICSI.
  • To determine the impact of protamine deficiency, acrosome integrity, and sperm morphology on ICSI outcomes.
  • To identify key sperm parameters influencing fertilization success after ICSI.

Main Methods:

  • Prospective study involving 68 infertile couples undergoing ICSI.

Related Experiment Videos

  • Semen analysis performed using World Health Organization criteria.
  • Assessment of protamine deficiency (CMA3 staining), acrosin activity (gelatinolysis test), and sperm morphology (Papanicolaou staining).

    • Main Results:

    • Protamine deficiency (indicated by CMA3 positivity) and acrosome size (halo diameter) significantly correlated with fertilization rates.
    • No significant correlation was observed between sperm normal morphology and fertilization rate.
    • Multiple linear regression identified protamine deficiency as the sole direct predictor of fertilization rate in ICSI.

    Conclusions:

    • Protamine deficiency is a critical factor affecting fertilization rates in ICSI.
    • Sperm DNA protamination is more influential than acrosin activity and conventional semen parameters for ICSI success.
    • Targeting protamine deficiency may improve ICSI outcomes in infertile males.