Related Experiment Video
Updated: Jul 14, 2026
High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
Quantification of pramipexole in human plasma by liquid chromatography tandem mass spectrometry using tamsulosin as
Ramakrishna V S Nirogi1, Vishwottam Kandikere, Wishu Shrivastava
1Biopharmaceutical Research, Suven Life Sciences Ltd, Serene Chambers, Road No. 5, Avenue 7, Banjara Hills, Hyderabad, India. Ramakrishna_nirogi@yahoo.co.in
Abstract:
A high-performance liquid chromatography/electrospray ionization tandem mass spectrometry method was developed and validated for the quantification of pramipexole in human plasma. Following liquid-liquid extraction, the analytes were separated using an isocratic mobile phase on a reverse-phase column and analyzed by MS/MS in the multiple reaction monitoring mode using the respective [M + H](+) ions, m/z 212/152 for pramipexole and m/z 409/228 for the IS. The method exhibited a linear dynamic range of 200-8000 pg/mL for pramipexole in human plasma. The lower limit of quantification was 200 pg/mL with a relative standard deviation of less than 8%. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. A run time of 3.5 min for each sample made it possible to analyze more than 200 human plasma samples per day. The validated method has been successfully used to analyze human plasma samples for application in pharmacokinetic, bioavailability or bioequivalence studies.
