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Updated: Jul 14, 2026

Rapid High-throughput Species Identification of Botanical Material Using Direct Analysis in Real Time High Resolution Mass Spectrometry
Published on: October 2, 2016
Cytotoxic withanolides from the flowers of Datura metel
Yuanhu Pan1, Xiaochuan Wang, Xianming Hu
1State Key Laboratory of Virology, College of Pharmacy, Wuhan University, Wuhan 430072, People's Republic of China.
Abstract:
Chemical investigation of a methanol extract of the flowers of Datura metel has led to the isolation of 10 new withanolides, withametelins I-P (1-8), 1,10-seco-withametelin B (9), and 12beta-hydroxy-1,10-seco-withametelin B (10), together with seven known withanolides. The structures of 1-10 were elucidated by means of spectroscopic methods, and the absolute stereochemistry of 1 was confirmed by single-crystal X-ray analysis. Compounds 1, 3, 4, and 6 exhibited cytotoxic activities against A549 (lung), BGC-823 (gastric), and K562 (leukemia) cancer cell lines, with IC50 values ranging from 0.05 to 3.5 microM.
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