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Microarray RNA/DNA in different stem cell lines

A C Piscaglia1, T Shupe, A Gasbarrini

  • 1Department of Pathology, Immunology and Laboratory Medicine, University of Florida, College of Medicine, Gainesville, FL 32610-0275, USA. piscag@pathology.ufl.edu

Current Pharmaceutical Biotechnology
|June 23, 2007
PubMed
Summary

Understanding the stem cell transcriptome is crucial for regenerative medicine and cancer research. This knowledge aids in maintaining stemness, understanding cell plasticity, and developing new cancer prevention strategies.

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DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...