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Updated: Jul 14, 2026
The MultiBac Protein Complex Production Platform at the EMBL
Published on: July 11, 2013
High-level bacterial expression and purification of apicomplexan micronemal proteins for structural studies
S Saouros1, T M A Blumenschein, K Sawmynaden
1Department of Biological Sciences, Division of Molecular Biosciences, Centre for Structural Biology, Imperial College London, South Kensington, London SW7 2AZ, UK.
Abstract:
The cysteine-rich N-terminal domain of the micronemal adhesive protein MIC1 (MIC1-NT) from Toxoplasma gondii was cloned, expressed in Escherichia coli and purified. MIC1-NT is amenable to structural studies as shown by preliminary NMR and X-ray analysis. Positive results with two further micronemal proteins indicate that our strategy has wider application.
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