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Updated: Jul 14, 2026

Measuring Plasma Membrane Protein Endocytic Rates by Reversible Biotinylation
Published on: December 23, 2009
Activity-dependent control of bulk endocytosis by protein dephosphorylation in central nerve terminals
Emma L Clayton1, Gareth J O Evans, Michael A Cousin
1Centre for Integrative Physiology, George Square, University of Edinburgh, Edinburgh, Scotland, UK.
Abstract:
Bulk endocytosis is the process by which nerve terminals retrieve large amounts of synaptic vesicle membrane during periods of strong stimulation intensity. The process is rapidly activated and is most probably calcium dependent in a similar manner to synaptic vesicle exocytosis. This article briefly summarizes the current knowledge of bulk endocytosis with respect to its activation, kinetics and molecular mechanism. It also presents recent data from our laboratory showing that the dephosphorylation of a group of endocytosis proteins called the dephosphins by the Ca(2+)-dependent protein phosphatase calcineurin is key to the activity-dependent stimulation of the process. Possible downstream effectors of calcineurin are discussed such as the large GTPase dynamin I and its phosphorylation-dependent interaction partner syndapin I.
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