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Serum Tamm-Horsfall glycoprotein level in children with various renal diseases
T Yamamoto1, H Miyata, T Fujiyama
1Department of Pediatrics, Kinki University School of Medicine, Osaka, Japan.
Insights
Serum Tamm-Horsfall glycoprotein (THGP) levels are often lower in patients with renal disease, correlating with decreased creatinine clearance. This suggests THGP measurement aids renal function evaluation.
Area of Science:
- Biochemistry
- Nephrology
- Clinical Chemistry
Background:
- Tamm-Horsfall glycoprotein (THGP) is a major urinary protein.
- Assessing renal function is crucial for managing kidney diseases.
Purpose of the Study:
- To investigate serum THGP concentrations in pediatric patients with various renal diseases.
- To evaluate the utility of serum THGP as a biomarker for renal function.
Main Methods:
- A competitive enzyme-linked immunosorbent assay (ELISA) was employed to measure serum THGP.
- 168 patients with renal diseases and 32 healthy controls were analyzed.
Main Results:
- Serum THGP levels were generally lower in patients with renal diseases compared to controls.
- A significant inverse correlation was observed between serum THGP levels and endogenous creatinine clearance (CCR).
- Three patients with vesicoureteric reflux exhibited elevated serum THGP levels, potentially due to urinary backflow.
Conclusions:
- Serum THGP measurement can be a valuable tool for assessing renal function in pediatric patients.
- Elevated serum THGP may indicate urinary backflow in specific conditions like vesicoureteric reflux.
Abstract:
Serum Tamm-Horsfall glycoprotein (THGP) concentrations were measured by a competitive enzyme-linked immunosorbent assay using peroxidase-labeled THGP in 168 patients, aged 10.5 +/- 4.6 years, with various renal diseases. Using this method, THGP was determined in the concentration range of 10-10(3) micrograms/l. Serum THGP levels ranged from 130 to 350 micrograms/l in 32 control subjects, aged 1-16 years with normal renal function. In most patients with renal disease, the serum THGP levels were lower than those in control subjects. In particular, the serum THGP levels were more reduced according to the decrease in the clearance values of endogenous creatinine (CCR). These findings suggested that the measurement of serum THGP levels is helpful in the evaluation of renal function. On the other hand, 3 patients with vesicoureteric reflux showed higher serum THGP levels than control subjects, though the CCR values in 1 of these patients was lower. These high serum THGP levels may be the result of urinary backflow into circulation.