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Simultaneous determination of 17 ginsenosides in rat urine by ultra performance liquid chromatography-mass
Xiaoyan Wang1, Tie Zhao, Xianfu Gao
1School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
Abstract:
A rapid analytical method for quantifying 17 ginsenosides in rat urine by ultra performance liquid chromatography (UPLC) coupled to electrospray ionization mass spectrometry (ESI-MS) is described. All analytes were extracted by solid-phase extraction optimized to obtain good recovery and quantified using digoxin as an internal standard. ESI-MS was optimized for different cone voltages at positive ionization mode to allow simultaneous analysis of all analytes in a relatively short time. Qualitative methodological considerations, including the linear range, precision, limit of quantification, limit of detection, recovery and sensitivity are also provided.
