Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Laser-based measurements in cell biology.

Elliot L Botvinick1, Jagesh V Shah

  • 1Beckman Laser Institute, Department of Biomedical Engineering, University of California, Irvine, California 92612, USA.

Methods in Cell Biology
|June 26, 2007
PubMed
Summary

This chapter explores laser-based microscopy techniques for molecular interrogation. It covers advanced imaging methods and biophysical parameter measurements in living cells.

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

686
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
686
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.5K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Uniform pore morphology of bijel-templated materials reduces cell circularity and inflammatory expression of macrophages.

Materials today. Bio·2026
Same author

Virtual fluorescent labeling of engineered vascular networks with embedded tracer particles.

Acta biomaterialia·2026
Same author

Active Microrheology Reveals Distinct ECM Mechanical Signatures Induced by Stromal Cells of Different Tissue Origins during Vascular Morphogenesis.

ACS biomaterials science & engineering·2026
Same author

Scanning Laser Cavitation Rheology for mesoscopic mapping of shear moduli of soft materials at high strain rates.

Acta biomaterialia·2026
Same author

Bioluminescent Immunophage Sensors for the Quantification of Insulin.

ACS omega·2026
Same author

Pore morphology of bijel-templated materials promotes migration and downregulates αSMA expression in human fibroblasts.

Frontiers in bioengineering and biotechnology·2026

Area of Science:

  • Optics and Photonics
  • Biophysics
  • Cell Biology

Background:

  • Laser illumination offers significant advantages for exciting fluorescence in microscopy.
  • Traditional laser microscopy techniques include confocal, multiphoton, and total internal reflection microscopy.
  • Emerging modalities leverage intrinsic macromolecular properties for imaging.

Purpose of the Study:

  • To review laser-based microscopy techniques for molecular interrogation.
  • To introduce advanced imaging modalities and super-resolution methods.
  • To explain laser-based techniques for measuring biophysical parameters in living cells.

Main Methods:

  • Confocal microscopy
  • Multiphoton microscopy
  • Total internal reflection microscopy
  • Second harmonic generation imaging
  • Coherent anti-Raman resonance spectroscopy
  • Super-resolution microscopy
  • Photobleaching techniques
  • Fluorescence lifetime imaging
  • Fluorescence correlation spectroscopy

Main Results:

  • Laser-based microscopy enables detailed molecular interrogation.
  • Advanced techniques provide super-resolution imaging beyond diffraction limits.
  • Biophysical parameters like kinetic rates, diffusion, and molecular interactions can be measured.

Conclusions:

  • Laser microscopy offers a versatile toolkit for biological research.
  • Readers can match specific research questions to appropriate laser-based techniques.
  • This field provides powerful methods for studying molecular behavior in living systems.

Related Experiment Videos