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Related Experiment Videos

Murine trefoil factor 3 does not directly modulate LPS-mediated dendritic cell function.

M Loos1, A De Creus1, L Thim1

  • 1Department for Molecular Biomedical Research, VIB, Ghent, BelgiumDepartment of Molecular Biology, Ghent University, Ghent, BelgiumDepartment of Protein Chemistry, Novo Nordisk, Bagsvaerd, Denmark.

Scandinavian Journal of Immunology
|June 26, 2007
PubMed
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Murine trefoil factor 3 (mTFF3) does not directly regulate dendritic cell (DC) function. Studies show mTFF3-treated DCs are phenotypically and functionally similar to untreated DCs, suggesting no impact on immune responses.

Area of Science:

  • Immunology
  • Gastroenterology
  • Molecular Biology

Background:

  • Trefoil factor family (TFF) peptides protect gastro-intestinal mucous epithelia and aid repair.
  • TFF2 and TFF3 are implicated in immune response regulation.
  • Dendritic cells (DCs) dictate immune tolerance or activation.

Purpose of the Study:

  • To investigate if murine TFF3 (mTFF3) directly influences dendritic cell (DC) function.
  • To compare LPS-induced maturation of mTFF3-treated DCs versus untreated DCs.

Main Methods:

  • Murine dendritic cells were treated with mTFF3 or left untreated.
  • Lipopolysaccharide (LPS) was used to induce DC maturation.
  • Phenotypic analysis and cytokine expression were assessed.
  • T-cell stimulation assays were performed.

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Main Results:

  • mTFF3-treated DCs and untreated DCs showed no phenotypic differences.
  • Co-stimulatory molecule and cytokine expression levels were similar in both groups, pre- and post-LPS stimulation.
  • Both DC populations efficiently stimulated T-cells.

Conclusions:

  • Murine TFF3 does not appear to directly regulate DC function.
  • mTFF3 does not govern immune responses at the DC functional level.