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Updated: Jul 14, 2026

An Ectopic Chemokine Expression Model for Testing Macrophage Recruitment In Vivo
Published on: September 25, 2019
LPS induces gene expression of interleukin-1beta in conger eel (Conger myriaster) macrophages: first cytokine
Shigeyuki Tsutsui1, Taiga Iwamoto, Osamu Nakamura
1School of Fisheries Science, Kitasato University, Ofunato, Iwate, Japan. tsutsui@kitasato-u.ac.jp
Abstract:
Our aim was to comprehensively analyze the immune response in Anguilliformes macrophages and to survey cytokine genes expressed from them. We therefore used suppression subtractive hybridization (SSH) to randomly clone molecules that are specifically expressed in conger eel (Conger myriaster) macrophages when cells are stimulated by LPS. As a result, we succeeded in identifying a conger eel IL-1beta. This is the first report on cytokines in Anguilliformes, which is the most ancient order in living teleosts.

