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Updated: Jul 14, 2026

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
Published on: July 8, 2025
Transcriptome analysis device based on liquid phase detection by fluorescently labeled nucleic acid probes
Ryuji Yokokawa1, Soichiro Tamaoki, Takashi Sakamoto
1Department of Microsystem Technology, Ritsumeikan University, 1-1-1 Noji-higashi, Kusatsu, Shiga, Japan. ryuji@se.ritsumei.ac.jp
Abstract:
Personalized medicine based on genetic information has been proposed as an attractive medical treatment. It is supported by the rapid development of the gene diagnosis utilizing on-chip analysis technology. This study reports characterizations of mRNA detection device by its hybridization with 2'-O-methyl oligoribonucleotide probe in a liquid phase, which eliminates time-consuming processes such as removing non-hybridized probes in conventional solid phase detection. In order to achieve a high sensitivity in the fluorescent detection of the target mRNA, we have optimized detection channel depth and width for a microfluidic device, and investigated polydimethyl siloxiane components and observation setup to minimize background noise. Adopting optimized channel dimensions and setup for the probe, the fluorescent intensity increased 3.3-fold at the lowest detectable concentration of 7.8 nM.
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