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Updated: Jul 14, 2026
Systems Analysis of the Neuroinflammatory and Hemodynamic Response to Traumatic Brain Injury
Published on: May 27, 2022
Cysteinyl leukotriene receptor 1 partially mediates brain cryoinjury in mice
Qian Ding1, San-hua Fang, Yu Zhou
1Department of Pharmacology, School of Medicine, Zhejiang University, Hangzhou 310058, China.
Aim:
To determine whether the cysteinyl leukotriene receptor 1 (CysLT1 receptor) modulates brain cryoinjury and whether the CysLT1 receptor antagonist pranlukast exerts a time-dependent protective effect on cryoinjury in mice.
Methods:
Brain cryoinjury was induced by applying a liquid nitrogen-cooled metal probe to the surface of the skull for 30 s. Brain lesion, neuron density, and endogenous IgG exudation were observed 24 h after cryoinjury. Transcription and the expression of the CysLT1 receptor were detected by RT-PCR and immunoblotting, and the localization of the receptor protein by double immunofluorescence.
Results:
The mRNA and protein expressions of the CysLT1 receptor were upregulated in the brain 6-24 h after cryoinjury, and the CysLT1 receptor protein was primarily localized in the neurons, not in the astrocytes or microglia. Pre-injury treatments with multi-doses and a single dose of pranlukast (0.1 mg/kg) attenuated cryoinjury; postinjury single dose (0.1 mg/kg) at 30 min (not 1 h) after cryoinjury was also effective.
Conclusion:
The CysLT1 receptor modulates cryoinjury in mice at least partly, and postinjury treatment with its antagonist pranlukast exerts the protective effect with a therapeutic window of 30 min.
Insights
The cysteinyl leukotriene receptor 1 (CysLT1 receptor) plays a role in brain cryoinjury. Its antagonist, pranlukast, shows a protective effect, particularly when administered within 30 minutes post-injury.
Area of Science:
- Neuroscience
- Immunology
- Pharmacology
Background:
- Brain cryoinjury is a significant research model for studying neurological damage.
- The cysteinyl leukotriene receptor 1 (CysLT1 receptor) is implicated in inflammatory processes.
Purpose of the Study:
- To investigate the role of the CysLT1 receptor in modulating brain cryoinjury.
- To evaluate the time-dependent protective effects of the CysLT1 receptor antagonist, pranlukast, in a mouse model.
Main Methods:
- Brain cryoinjury was induced using a standardized liquid nitrogen-cooled probe.
- CysLT1 receptor expression (mRNA and protein) and localization were analyzed post-injury.
- The effects of pre- and post-injury administration of pranlukast on brain lesion, neuron density, and IgG exudation were assessed.
Main Results:
- CysLT1 receptor expression was upregulated in the brain following cryoinjury.
- The CysLT1 receptor protein was predominantly found in neurons.
- Pranlukast administration, both pre- and post-injury (within 30 minutes), attenuated cryoinjury.
Conclusions:
- The CysLT1 receptor is involved in modulating brain cryoinjury.
- Post-injury treatment with pranlukast offers a protective effect within a 30-minute therapeutic window.
