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Updated: Jul 14, 2026
T4 Bacteriophage and E. coli Interaction in the Murine Intestine: A Prototypical Model for Studying Host-Bacteriophage Dynamics In Vivo
Published on: January 26, 2024
Purification and concentration of bacteriophage T4 using monolithic chromatographic supports
F Smrekar1, M Ciringer, M Peterka
1BIA Separations d.o.o., Teslova 30, SI-1000 Ljubljana, Slovenia.
Abstract:
Phages are gaining importance due to their wide usage. In this work strong anion exchange monolithic chromatographic column was used for single step phage purification. Most of the proteins and DNA were removed and recovery of approximately 70% of infective virus was reproducibly achieved. 30 ml of phage sample was purified in around 10 min.
Insights
This study demonstrates a rapid, single-step purification of bacteriophages using strong anion exchange chromatography. This method efficiently removes contaminants and recovers approximately 70% of infectious virus particles.
Area of Science:
- Biotechnology
- Virology
- Chromatography
Background:
- Bacteriophages (phages) are increasingly utilized in various applications.
- Efficient purification methods are crucial for phage applications.
Purpose of the Study:
- To develop a single-step purification method for bacteriophages.
- To assess the efficiency of strong anion exchange monolithic chromatography for phage purification.
Main Methods:
- Utilized a strong anion exchange monolithic chromatographic column.
- Purified 30 ml of phage sample in approximately 10 minutes.
Main Results:
- Achieved reproducible recovery of approximately 70% infective virus.
- Successfully removed most contaminating proteins and DNA.
- Demonstrated a rapid purification process.
Conclusions:
- Strong anion exchange monolithic chromatography is an effective method for single-step phage purification.
- This technique offers high recovery rates and efficient contaminant removal.
- The method is time-efficient for purifying large sample volumes.
