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A Simple Microaspiration Technique for Isolating Somatic Cells from Cryopreserved Equine Semen as Nuclear Donors for Cloning
Published on: December 19, 2025
The efficient use of equine cryopreserved semen
1Honahlee PC, Sherwood, OR, USA. honahlee@imagina.com
Abstract:
In order to optimize the efficient use of cryopreserved stallion semen, recent research has focused on the minimum insemination dose of frozen-thawed spermatozoa required for maximum fertility rate. The results appear to be highly stallion-dependent. Factors such as the timing of AI with respect to ovulation, as well as the site of insemination within the mare's reproductive tract, also affect success in breeding with frozen-thawed semen. Since acceptable pregnancy rates can be achieved from insemination of mares with very low numbers of spermatozoa, increasing the number of insemination doses processed from a single ejaculate may prove more cost-effective to stallion owners.

