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Source And Potency Of Stem Cells01:27

Source And Potency Of Stem Cells

Stem cells are undifferentiated cells with extensive self-renewal properties that help them maintain their population during the fetal and adult stages of life. They can specialize in all cell types of the human body. However, their differential potential may vary and can be classified into five types. Stem cells can be (1) Totipotent, (2) Pluripotent, (3) Multipotent, (4) Oligopotent, and (5) Unipotent. Each stem cell has a specific origin; the fertilized egg or zygote is a totipotent cell and...
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Generation of Myospheres From hESCs by Epigenetic Reprogramming
09:32

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Published on: June 21, 2014

Clonal multipotency of skeletal muscle-derived stem cells between mesodermal and ectodermal lineage.

Tetsuro Tamaki1, Yoshinori Okada, Yoshiyasu Uchiyama

  • 1Muscle Physiology and Cell Biology Unit, Department of Regenerative Medicine, Division of Basic Clinical Science, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa 259-1143, Japan. tamaki@is.icc.u-tokai.ac.jp

Stem Cells (Dayton, Ohio)
|June 26, 2007
PubMed
Summary

Single skeletal muscle-derived double negative (Sk-DN) cells show clonal multipotency, differentiating into myogenic, vasculogenic, and neural lineages. This discovery offers potential for regenerating damaged muscle tissue.

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14:47

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Published on: May 17, 2016

Area of Science:

  • Stem Cell Biology
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Skeletal muscle-derived stem cells (MDSCs) are known for differentiation potential.
  • The clonal multipotency of MDSCs requires further investigation.

Purpose of the Study:

  • To determine the clonal multipotency of single skeletal muscle-derived CD34-/CD45- (Sk-DN) cells.
  • To assess the differentiation capacity of Sk-DN cells into myogenic, vasculogenic, and neural lineages.

Main Methods:

  • Single Sk-DN cells were isolated from GFP transgenic mice and cultured.
  • Clonal colony-forming units (CFUs) were transplanted into damaged wild-type mouse muscle.
  • Differentiation was analyzed using marker expression (mRNA) and cell lineage identification.

Main Results:

  • Single Sk-DN cells demonstrated clonal multipotency in vitro and in vivo.
  • Transplanted cells differentiated into endothelial, vascular smooth muscle, skeletal muscle, and neural (Schwann) cells.
  • Approximately 70% of clonal CFUs expressed markers for all three lineages.

Conclusions:

  • Sk-DN cells possess significant clonal multipotency.
  • These cells can contribute to synchronized myogenesis, vasculogenesis, and neurogenesis.
  • Sk-DN cells represent a promising tool for muscle-related tissue repair.