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Updated: Jul 14, 2026

Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay
Published on: June 9, 2011
[Presence of west Nile virus in northeast Mexico]
Ildefonso Fernández-Salas1, María de Lourdes Garza-Rodríguez, Barry J Beaty
1Laboratorio de Entomología Médica, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, México. ifernand@ccr.dsi.uanl.mx
Objective:
To investigate the presence of WNV in birds, horses and humans in northeast Mexico.
Material And Methods:
Serum samples from 33 birds, 24 horses and 237 humans were screened by ELISA for Anti-WNV antibodies. Human serum samples were also screened for WNV RNA using an RT-PCR assay.
Results:
Positive sera were found in three birds and 15 horses. Forty percent of the human serum samples were positive for IgG antibodies and 0% for IgM antibodies and viral RNA.
Conclusions:
The results of this study show that WNV is present in northeast Mexico and it is a new emergent infectious agent that represents a challenge for research and prevention programs in Mexico.
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