Ligand-induced extramembrane conformation switch controlling alamethicin assembly and the channel current
1Institute for Chemical Research, Kyoto University, Uji, Kyoto, Japan. futaki@scl.kyoto-u.ac.jp
Abstract:
In this review, we describe our approach to creating artificial receptor-channel proteins or sensor systems, using an extramembrane segment conformationally switchable by external stimuli. Alamethicin is known to self-assemble in membranes to form ion channels with various open states. Employment of an alpha-helical leucine-zipper segment resulted in the effective modulation of the association states of alamethicin to produce a single predominant channel-open state. A decrease in the helical content of the extramembrane segments was found to induce a channel-current increase. Therefore, conformational changes in the extramembrane segments induced by the interaction with ligands can be reflected in the current levels.
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