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Updated: Jul 14, 2026

A11-positive β-amyloid Oligomer Preparation and Assessment Using Dot Blotting Analysis
Published on: May 22, 2018
Inhibition of beta-amyloid aggregation and neurotoxicity by complementary (antisense) peptides
Jonathan R Heal1, Gareth W Roberts, Gary Christie
1Imperial College Genetic Therapies Centre Department of Chemistry, Flowers Building, Armstrong Road Imperial College of Science, Technology and Medicine South Kensington, London, SW72AZ, UK.
Abstract:
Complementary peptides are coded for by the nucleotide sequence (read 5'-->3') of the complementary strand of DNA. By reading the sequence of complementary DNA in the 3'-->5' direction, alternative complementary peptides may be derived. We describe the derivation, testing and analysis of six complementary peptides designed against beta-amyloid peptide 1-40 (Abeta, 40). Data is presented to show that one peptide, designated 3' -->5' betaCP1-15, binds specifically to Abeta 1-40, and inhibits both fibrilisation and neurotoxicity in vitro. This suggests that complementary peptides could be useful leads for drug discovery, especially where diseases of protein misfolding are concerned.
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