Related Experiment Video
Updated: Jan 26, 2026

Deciphering the Molecular Mechanism and Function of Pore-Forming Toxins Using Leishmania major
Published on: October 28, 2022
[Preparation of fast responsive, pH sensitive polyacrylic acid gel with different pore-forming agents]
1College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
Abstract:
Five kinds of pore-forming agents, including PEG-400, chitosan, the polymer of chitosan and glutin, CaCO3 and NazCO3, were used in this experiment to prepare fast responsive and pH sensitive Polyacrylic acid (PAAc) gel which could be used as the material of punctum plug. The mechanism and effects of pore-forming agents on PAAc gel were studied; besides, the experiment also assessed the effects of the three most effective pore-forming agents on gel's water retention and swelling rate. The results showed that the most suitable dose levels of PEG-400, the polymer and Na2CO3 were 1 ml, 0.8 ml and 1 ml respectively, and PEG-400 was most effective with the same dosage; the gel with PEG-400 as pore-forming agent could finish more than 95% of the swelling process in just 5 minutes, it is suitable for use as punctum plug material.
Related Concept Videos
Preparation of Acid Anhydrides
The carboxylate ion acts as a nucleophile that attacks the carbonyl carbon of the acid chloride to form a tetrahedral intermediate. Subsequently, the re-formation of the carbonyl group with the loss of the chloride ion as a leaving group leads to the formation of an acid...
Preparation of Carboxylic Acids: Overview
Sputum Studies I: Gram Stain, cytology, and Acid-fast smear and culture
Gram Stain
The Gram Stain is an integral part of sputum studies. It involves the staining of sputum, which permits...
Preparation of Carboxylic Acids: Hydrolysis of Nitriles
Pore Size Distribution
Adequate...
Amino acids

