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Related Experiment Videos

Serum response factor contributes selectively to lymphocyte development.

Anne Fleige1, Siegfried Alberti, Lothar Gröbe

  • 1Department of Experimental Immunology, Helmholtz Centre for Infection Research, 38124 Braunschweig, Germany.

The Journal of Biological Chemistry
|June 27, 2007
PubMed
Summary
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Serum response factor (SRF) is vital for T cell development, causing a block in thymocyte maturation. SRF also impacts B cell development, affecting marginal zone and CD5+ subsets.

Area of Science:

  • Immunology
  • Molecular Biology
  • Developmental Biology

Background:

  • Serum response factor (SRF) is essential for embryonic development, muscle, and neuron function.
  • SRF and cofactors are expressed in lymphocytes, but its role in lymphocyte development is unknown.
  • Conventional Srf-null mice exhibit embryonic lethality, preventing in vivo study of SRF in lymphocytes.

Purpose of the Study:

  • To investigate the in vivo role of SRF in T and B lymphocyte development.
  • To overcome the embryonic lethality issue using lymphocyte-specific gene inactivation.

Main Methods:

  • Utilized lymphocyte-specific Cre transgenic mouse lines to specifically inactivate the murine Srf gene.
  • Analyzed thymocyte and peripheral lymphocyte populations following Srf deletion in T and B cell lineages.

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Main Results:

  • T cell-specific Srf deletion caused a severe block in thymocyte development at the double to single positive stage.
  • B cell-specific Srf deletion did not affect general B cell survival but eliminated marginal zone B cells and reduced CD5+ B cells.
  • SRF influences the expression of IgM, CD19, and chemokine receptor 4 in B lymphocytes.

Conclusions:

  • SRF plays critical and distinct roles in the development of T and B lymphocytes.
  • SRF is indispensable for normal thymocyte maturation.
  • SRF regulates specific B cell subsets and surface molecule expression.