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Related Experiment Video

Updated: Jul 14, 2026

A Method For Production of Recombinant mCD1d Protein in Insect Cells.

A Method For Production of Recombinant mCD1d Protein in Insect Cells.

Published on: December 10, 2007

Architecture of CD1 proteins.

D M Zajonc¹, I A Wilson

¹Department of Molecular Biology, Skaggs Institute of Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.

Current Topics in Microbiology and Immunology

|June 28, 2007

Summary

The CD1 family of cell surface receptors presents lipid antigens to T cells. Structural differences in how CD1 group 1 and group 2 present lipids impact T cell activation and drug design.

Area of Science:

Immunology
Structural Biology
Biochemistry

Background:

The CD1 family of glycosylated cell surface receptors plays a crucial role in presenting lipid antigens to T cells.
Understanding the structural basis of CD1-lipid interactions is key to deciphering T cell recognition and activation pathways.

Purpose of the Study:

To elucidate the distinct structural mechanisms by which CD1 group 1 and group 2 isoforms present lipid antigens.
To investigate ligand-induced conformational changes in CD1 molecules upon antigen binding.

Main Methods:

X-ray crystallography was employed to determine the structures of CD1-lipid complexes.
Comparative structural analysis of CD1 group 1 (CD1a, CD1b, CD1c) and group 2 (CD1d) isoforms bound to various lipid antigens.

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Last Updated: Jul 14, 2026

A Method For Production of Recombinant mCD1d Protein in Insect Cells.

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Published on: December 10, 2007

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

Published on: April 23, 2017

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation

Published on: February 28, 2019

Main Results:

CD1 group 1 isoforms anchor the lipid backbone within hydrophobic grooves, while CD1d (group 2) positions polar headgroups via hydrogen bonding.
CD1d exhibits ligand-induced conformational changes upon binding of alpha-galactosylceramide, involving polar interactions with helices.
No significant ligand-induced conformational changes were observed for group 1 CD1 complexes with diverse lipid antigens.

Conclusions:

Distinct structural modes of lipid presentation by CD1 isoforms dictate T cell recognition.
Ligand-induced conformational changes in CD1d offer insights into invariant NKT cell activation.
Structural data facilitate the rational design of novel immunomodulatory agents and therapeutics for immune-mediated diseases.