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Related Concept Videos

Genetic Screens02:46

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Gene suppression technologies in high-throughput analysis: front- and back-side applications.

Mark Laflamme1, Gilles A Robichaud

  • 1Atlantic Cancer Research Institute, Moncton, New Brunswick, Canada. Markl@canceratl.ca

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|June 28, 2007
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Summary

High-throughput systems and RNA-based gene suppression accelerate the study of gene function and interactions. Combining these methods with DNA microarrays provides vast data for molecular pathway analysis.

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Area of Science:

  • Molecular Biology
  • Genomics
  • Systems Biology

Background:

  • Gene function and interactions are complex, involving multiple partners and pathways.
  • Traditional methods for studying gene function include animal knockouts and natural mutants.
  • High-throughput systems have revolutionized the understanding of gene interactions.

Purpose of the Study:

  • To review gene inactivation systems suitable for molecular pathway analysis.
  • To highlight the synergy between RNA-based gene suppression and microarray analysis.
  • To discuss the advantages of RNA-based systems over traditional methods.

Main Methods:

  • Review of RNA-based gene suppression techniques (e.g., RNA interference, ribozymes).
  • Integration of gene inactivation with genome-wide expression analysis using DNA microarrays.
  • Analysis of data generated from combined high-throughput systems.

Main Results:

  • RNA-based gene suppression offers ease of use, specificity, and broad applicability.
  • The combination of gene suppression and molecular profiling yields extensive data.
  • Rapid advancements in understanding gene interactions and function are occurring.

Conclusions:

  • RNA-based gene inactivation systems are valuable tools for molecular pathway analysis.
  • Synergistic use of gene suppression and microarray analysis enhances biological insights.
  • High-throughput approaches are crucial for deciphering complex gene networks.