Related Experiment Video
Updated: Jul 14, 2026

Detection of Live Escherichia coli O157:H7 Cells by PMA-qPCR
Published on: February 1, 2014
Persistence of Shiga toxin-producing Escherichia coli O26 in cow slurry
B Fremaux1, C Prigent-Combaret, M L Delignette-Muller
1Unité de Microbiologie Alimentaire et Prévisionnelle-Ecole Nationale Vétérinaire de Lyon 69280, Marcy l'Etoile, France. b.fremaux@vet-lyon.fr
Aims:
The main objective of this study was to evaluate the growth and survival of Shiga toxin-producing Escherichia coli (STEC) O26 in cow slurry; this serogroup is regarded as an important cause of STEC-associated diseases.
Methods And Results:
Four STEC were examined by polymerase chain reaction (PCR) to determine whether they harbour key virulence determinants and also by pulsed-field gel electrophoresis (PFGE) to obtain overview fingerprints of their genomes. They were transformed with the pGFPuv plasmid and were separately inoculated at a level of 10(6) CFU ml(-1) in 15 l of cow slurry. All STEC O26 strains could be detected for at least 3 months in cow slurry without any genetic changes. The moisture content of the slurry decreased over time to reach a final value of 75% while the pH increased from 8.5 to 9.5 units during the last 50 days.
Conclusion:
STEC O26 strains were able to survive in cow slurry for an extended period.
Significance And Impact Of The Study:
Long-term storage of waste slurry should be required to reduce the pathogen load and to limit environmental contamination by STEC O26.
More Related Videos
09:44Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
Published on: December 23, 2022
06:20Detection of Enterohemorrhagic Escherichia Coli Colonization in Murine Host by Non-invasive In Vivo Bioluminescence System
Published on: April 9, 2018
Related Concept Videos
Bacterial Gastroenteritis
Stringent Response in E. coli
Investigation of Disease Outbreaks
Clinical Significance of Antibiotic Resistance
Cholera
Sources of Food Contamination