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Published on: March 17, 2023
Profiling of chicken adipose tissue gene expression by genome array
Hong-Bao Wang1, Hui Li, Qi-Gui Wang
1College of Animal Science and Technology, Northeast Agricultural University, Harbin, PR China. wanghongbao001@yahoo.com.cn <wanghongbao001@yahoo.com.cn>
BMC Genomics
|June 28, 2007
Summary
This study profiles chicken adipose tissue gene expression to understand obesity. Key genes involved in lipid metabolism and immunity were identified, aiding future research into chicken fatness.
Area of Science:
- Animal Science
- Genomics
- Molecular Biology
Background:
- Excessive lipid accumulation in broiler adipose tissue is a significant industry concern.
- Limited research exists on adipose tissue gene expression related to chicken adiposity.
- Gene expression profiling can elucidate fatness ontogenesis and obesity mechanisms.
Purpose of the Study:
- To construct an adipose tissue gene expression profile in 7-week-old broilers.
- To identify differentially expressed genes between lean and fat chicken lines.
- To understand the molecular mechanisms underlying obesity in chickens.
Main Methods:
- Utilized Chicken Genome Arrays for gene expression profiling.
- Divergent selection of broilers for high and low abdominal fat weight over eight generations.
- Validated differentially expressed genes using real-time RT-PCR.
Main Results:
- Detected 13,234-16,858 probe sets, with high expression of lipid metabolism and immunity genes (e.g., FABP, LPL, MHC).
- Some lipogenesis genes (e.g., leptin receptor, SREBP1) were not detected.
- Screened 230 differentially expressed genes, primarily in lipid metabolism, signal transduction, energy metabolism, tumorigenesis, and immunity.
Conclusions:
- Established a foundation for studying genetic control of chicken adipose tissue growth and development.
- Provides insights into the molecular mechanisms of obesity in chickens.
- Highlights the role of specific genes in lipid metabolism and immunity related to fatness.
