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Updated: Jul 14, 2026

Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death
Published on: December 27, 2016
Signalling events employed in the hypertonic activation of cation channels in HeLa cells
Frank Wehner1, Tomohiro Numata, Muthangi Subramanyan
1Department of Cell Physiology, National Institute for Physiological Sciences, Okazaki, Japan.
Abstract:
In the present study, the signalling network behind the hypertonic activation of cation channels in HeLa cells was analysed by use of various inhibitors. Channel activation was monitored in whole-cell patch-clamp recordings, whereas the role of the channel in cell volume regulation was determined by electronic cell sizing. It is found that channel activation and volume control probably employs tyrosine kinases, G-proteins, PLC, PKC and p38MAP kinase, and that the process appears to depend on an intact actin skeleton. In contrast, RhoA, PI 3-kinase, ERK 1/2, JNK 1/2 as well as the exocytotic insertion of channels into the plasma membrane are likely not part of the signalling machinery.
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