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Updated: Jul 14, 2026

Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis
07:42

Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis

Published on: November 26, 2015

A variant epidermal growth factor receptor protein is similarly expressed in benign hyperplastic and carcinomatous

E O Olapade-Olaopa1, D K Moscatello, E H MacKay

  • 1Department of Urology, Leicester General Hospital, Leicester, UK. okeoffa@yahoo.com

West African Journal of Medicine
|June 29, 2007
PubMed
Abstract

Insights

Epidermal Growth Factor Receptor variant III (EGFRvIII) expression in prostate cancer is similar in black and white men. This suggests that EGFRvIII-targeted therapies may be equally effective across diverse populations.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Anti-epidermal growth factor receptor (EGFR) strategies are established cancer treatments.
  • EGFRvIII, a variant EGFR, is a target for prostate cancer treatments.
  • Previous studies identified EGFRvIII in UK white men but not in black men.

Purpose of the Study:

  • To determine EGFRvIII expression in prostatic tissues from Nigerian (black) and UK white men.
  • To compare EGFRvIII expression levels in normal, benign hyperplastic (BPH), and carcinomatous (CaP) prostate tissues between ethnic groups.

Main Methods:

  • Utilized streptavidin immunohistochemical techniques on archival prostatic tissue sections.
  • Employed a semi-quantitative visual scoring method for EGFRvIII immunoreactivity.
  • Performed statistical comparisons of EGFRvIII expression between normal, BPH, and CaP tissues in both populations.

Main Results:

  • EGFRvIII expression significantly increased with increasing prostatic malignancy (CaP > BPH > Normal; p < 0.0001) in both populations.
  • EGFRvIII expression levels were statistically similar in both benign hyperplastic and carcinomatous prostate tissues between black and white men (p = 0.86 and p = 0.31, respectively).

Conclusions:

  • EGFRvIII immunoreactivity in prostate tumors is comparable between black and white men.
  • Anti-cancer treatments targeting EGFRvIII are expected to demonstrate similar efficacy in both subpopulations.

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