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Related Experiment Videos

KGF promotes integrin alpha5 expression through CCAAT/enhancer-binding protein-beta.

Piyush Koria1, Stelios T Andreadis

  • 1Bioengineering Laboratory, Dept. of Chemical and Biological Engineering, 908 Furnas Hall, Univ. at Buffalo, State Univ. of New York, Amherst, NY 14260, USA.

American Journal of Physiology. Cell Physiology
|June 29, 2007
PubMed
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Keratinocyte growth factor (KGF) upregulates alpha(5)beta(1)-integrin during wound healing by activating the C/EBP transcription factor. This mechanism is crucial for epidermal repair and may be better studied in 3D tissue models.

Area of Science:

  • Dermatology
  • Molecular Biology
  • Cell Biology

Background:

  • Keratinocyte growth factor (KGF) and alpha(5)beta(1)-integrin are upregulated in migrating epidermis during wound healing.
  • Their expression is absent in normal skin, highlighting their role in repair processes.

Purpose of the Study:

  • To investigate the mechanism by which KGF influences alpha(5)beta(1)-integrin expression.
  • To explore the role of transcription factors and signaling pathways in KGF-mediated integrin regulation.

Main Methods:

  • KGF treatment of epidermoid carcinoma cells and stratified bioengineered epidermis.
  • Analysis of alpha(5) mRNA and protein levels.
  • Promoter assays to identify transcription factor binding sites.
  • Western blotting for protein phosphorylation (ERK1/2, C/EBP-beta).

Related Experiment Videos

  • siRNA knockdown of C/EBP-beta.

    • Main Results:

    • KGF significantly increased alpha(5) mRNA and protein expression in both cell lines and engineered epidermis.
    • KGF-induced alpha(5) promoter activation was dependent on C/EBP transcription factor binding.
    • KGF promoted C/EBP-beta phosphorylation via ERK1/2 signaling.
    • Inhibition of C/EBP-beta blocked alpha(5) promoter activity and reduced integrin alpha(5) expression.
    • Upregulation of alpha(5) was more pronounced in 3D tissue models than 2D cultures.

    Conclusions:

    • KGF enhances alpha(5)beta(1)-integrin expression through the C/EBP-beta transcription factor pathway.
    • ERK1/2 signaling mediates KGF-induced C/EBP-beta phosphorylation.
    • Stratified epidermis and 3D models provide a more relevant context for studying growth factor effects in wound healing.