Changes in the immunoelectrophoretic characteristics of serum alpha-I-lipoprotein induced by various dyes

The effect of 8 dyes on the electrophoretic mobility of normal human serum alpha-1 lipoprotein has been examined. The two characteristics of increase in anodal mobility, and conformational changes of the immunoelectrophoretic precipitin arc suggesting polymorphism, were found to vary independently among the dyes tested. The results suggest that dye induced electrophoretic mobility alteration may represent a useful tool for examination of heterogeneity of alpha-1 lipoprotein.