Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 14, 2026

Microfluidic Fabrication of Core-Shell Microcapsules carrying Human Pluripotent Stem Cell Spheroids
10:51

Microfluidic Fabrication of Core-Shell Microcapsules carrying Human Pluripotent Stem Cell Spheroids

Published on: October 13, 2021

Nanoencapsulation of stem cells within polyelectrolyte multilayer shells.

Nalinkanth G Veerabadran1, Poorna L Goli, Skylar S Stewart-Clark

  • 1Institute for Micromanufacturing, Louisiana Tech University, 911 Hergot Avenue, Ruston, Louisiana 71272, USA.

Macromolecular Bioscience
|June 30, 2007
PubMed
Summary

Mouse mesenchymal stem cells were encapsulated in polyelectrolyte shells using layer-by-layer assembly. The cells maintained viability and morphology for one week, showing potential for tissue engineering applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hierarchical Oil-Water-Oil Pickering Double Emulsions Stabilized by Tubular Nanoparticles.

Journal of the American Chemical Society·2026
Same author

Correction to "Halloysite/polyaniline Nanocomposites for Enhanced Actinide Sorption".

ACS applied materials & interfaces·2026
Same author

Halloysite/polyaniline Nanocomposites for Enhanced Actinide Sorption.

ACS applied materials & interfaces·2025
Same author

Cellulose-Based Nanofibers in Wound Dressing.

Biomimetics (Basel, Switzerland)·2025
Same author

The Emerging Role of Halloysite Clay Nanotube Formulations in Cosmetics and Topical Drug Delivery.

ACS applied bio materials·2025
Same author

Fabrication and Characterization of a Stretchable Sodium Alginate Hydrogel Patch Combined with Silicon Nitride and Metalized Halloysite Nanotubes to Develop a Chronic Wound Healing Treatment.

International journal of molecular sciences·2025

Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Tissue Engineering

Background:

  • Mesenchymal stem cells (MSCs) are crucial for regenerative medicine.
  • Encapsulation techniques are needed to protect cells for therapeutic applications.

Purpose of the Study:

  • To develop a method for individually encapsulating mouse mesenchymal stem cells.
  • To assess the viability and morphology of encapsulated cells.

Main Methods:

  • Utilized the electrostatic layer-by-layer assembly technique.
  • Applied polyelectrolyte layers of poly(L-lysine) and hyaluronic acid.
  • Encased individual mouse mesenchymal stem cells.

Main Results:

  • Successfully created nanolayer shells around individual cells (6-9 nm thickness).

More Related Videos

Alginate Encapsulation of Pluripotent Stem Cells Using a Co-axial Nozzle
07:13

Alginate Encapsulation of Pluripotent Stem Cells Using a Co-axial Nozzle

Published on: July 2, 2015

Encapsulation Thermogenic Preadipocytes for Transplantation into Adipose Tissue Depots
08:30

Encapsulation Thermogenic Preadipocytes for Transplantation into Adipose Tissue Depots

Published on: June 2, 2015

Related Experiment Videos

Last Updated: Jul 14, 2026

Microfluidic Fabrication of Core-Shell Microcapsules carrying Human Pluripotent Stem Cell Spheroids
10:51

Microfluidic Fabrication of Core-Shell Microcapsules carrying Human Pluripotent Stem Cell Spheroids

Published on: October 13, 2021

Alginate Encapsulation of Pluripotent Stem Cells Using a Co-axial Nozzle
07:13

Alginate Encapsulation of Pluripotent Stem Cells Using a Co-axial Nozzle

Published on: July 2, 2015

Encapsulation Thermogenic Preadipocytes for Transplantation into Adipose Tissue Depots
08:30

Encapsulation Thermogenic Preadipocytes for Transplantation into Adipose Tissue Depots

Published on: June 2, 2015

  • Maintained cell morphology and viability for up to one week.
  • Demonstrated potential for tuning shell properties.

    • Conclusions:

    • Individual cell encapsulation using layer-by-layer assembly is feasible.
    • Encapsulated MSCs show promising viability for short-term applications.
    • Further optimization can enable tissue engineering and drug delivery uses.