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Preparation of monoclonal antibodies against the spindle checkpoint kinase Bub1

Rowena L Lock1, Tim H Szeto, Alan Entwistle

  • 1Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, London, United Kingdom.

Hybridoma (2005)
|June 30, 2007
PubMed

The Bub1 kinase is a critical component of the spindle checkpoint involved in monitoring the separation of sister chromatids at mitosis. The viral oncoprotein Simian virus 40 large T antigen (LT) can bind and perturb the spindle checkpoint function of Bub1. We have developed three highly specific monoclonal antibodies against the Bub1 protein and have demonstrated that they can all detect Bub1 via Western blotting and immunofluorescence, in addition to their ability to immunoprecipitate Bub1.

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