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Generation and characterization of a monoclonal IgG antibody to polyethylene glycol

David A Wunderlich1, Margit Macdougall, Diane V Mierz

  • 1Department of Research Technologies, Bayer Pharmaceuticals Corporation, West Haven, Connecticut 06516, USA.

Hybridoma (2005)
|June 30, 2007
PubMed

An IgG mouse monoclonal antibody (10F05) against polyethylene glycol has been generated. The antibody reacts with PEG regardless of the linker used for PEG attachment, and is able to recognize a PEGylated peptide in plasma at concentrations as low as 3 pg/mL. The antibody is readily purified in substantial quantities. The PEG IgG will find significant utility in the sensitive detection of PEG derivitives during the pharmacokinetic characterization of PEGylated compounds.

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Hybridoma Technology01:31

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Commonly used fusion techniques — electroporation, polyethylene glycol...