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Folylpolyglutamate synthetase from beef liver: purification and some properties
Z B Pristupa1, C M McAuley, K G Scrimgeour
1Department of Biochemistry, University of Toronto, Ont., Canada.
Abstract:
The polypeptide chain of folylpolyglutamate synthetase from beef liver has been isolated and partially characterized. This polypeptide has an apparent molecular weight of 73,000. Its amino-terminal residue is blocked. Amino acid analysis agrees with the hydrophobic properties and the pI (6.0) of this cytosolic enzyme. Polyclonal antibodies to the denatured enzyme have been prepared.