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Updated: Jul 14, 2026
Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation
Published on: July 3, 2017
Identification of vascular surface proteins by in vivo biotinylation: a method sufficiently sensitive to detect
Lauren A McCann1, Miriam C Haywood, Bin Hai Ren
1Bill Walsh Cancer Research Laboratories, Royal North Shore Hospital and University of Sydney, St. Leonards NSW 2065, Australia.
Abstract:
We have developed a methodology to selectively isolate and identify proteins associated with the luminal surface of blood vessels using in vivo biotinylation, streptavidin-affinity chromatography, and SDS-PAGE/LC-MS/MS. This had sufficient sensitivity to identify 32 proteins with changed expression in rat livers at 2 weeks or 5 weeks after partial hepatectomy, well after the 7 day tissue remodeling period. This method could be adapted to study other angiogenic tissues including tumors.
